Comprehensive transcriptome and metabolome profiling reveals key regulating signaling and metabolic pathways of resistant hypertension in drug users

Abstract

Abstract Background: Hypertension patients with drug use have a low susceptibility to hypotensor. The pathogenesis and metabolic characteristics of hypertension in drug users remain unknown. Methods: We performed high-throughput sequencing for transcriptome and metabolome of peripheral blood from 30 hypertension patients with drug use (HBP-DU) and 30 hypertension patients without drug use (HBP). Results: Differentially expressed genes (DEGs) between the HBP and HBP-DU groups were mainly involved in the pathways of toll-like receptor (TLR) cascades, FCERI signaling, and interleukin signaling. Differentially metabolites (DMs) were significantly enriched in the pathways of alanine and histidine metabolism. We identified a gene cluster (turquoise module, MEturquoise) correlated with the HBP-DU group, which has a similar function to DEGs. The abundance of CD4+ T cells and CD8+ T cells was significantly decreased in the HBP-DU group. Conclusions: These results indicated a potential function of histidine, immune cells, and the pathway of toll-like receptor cascades in drug use hypertension.