Exploration of the role of Mesencephalic astrocyte-derived neurotrophic factor in septic acute kidney injury

Abstract

Abstract Objective The Mesencephalic astrocyte-derived neurotrophic factor whether it has a protective effect on sepsis renal injury. Methods Mice were randomly divided into Control group, Control + MANF group, SAKI group, and SAKI + MANF group (n = 24). SAKI model mice were constructed by intraperitoneal injection of 10 mg/kg LPS to SAKI group and SAKI + MANF group mice. The WESTERN BLOT method and ELISA method were used to determine the change of MANF content in mice with septic kidney injury. Then the renal function and inflammatory factors of SAKI model mice were measured, and the relationship between MANF content and renal function and inflammatory factors were observed. Subsequently, we injected 200µg/Kg MANF into the abdominal cavity of mice in the Control + MANF group and SAKI + MANF group. Measure the change of MANF content in mice and its relationship with renal function and inflammatory factors. Finally, the kidneys of the four groups of mice were observed by preparing pathological sections of kidney tissues. Results In the early stage of sepsis renal injury, the content of MANF increased rapidly, but with the progress of the disease, the content of MANF decreased, the renal function of mice decreased, An obvious abnormality was found in all indexes. After recombinant human Mesencephalic astrocyte-derived neurotrophic factor was given to septic renal injury mice, the renal function was restored. Pathological sections showed that the renal tissue of normal + MANF mice was similar to that of normal mice, and there was no significant change. The renal tissue of septic renal injury mice was significantly damaged. Conclusion Mesencephalic astrocyte-derived neurotrophic factor has a certain protective effect on septic renal injury. Exogenous supplement of MANF can effectively alleviate the degree of renal injury after septic renal injury.