Affiliation:
1. Second Affiliated Hospital of Nanchang University
2. Nanchang university
Abstract
Abstract
Purpose
Hepatocellular carcinoma (HCC) is a prevalent tumor with high morbidity, and an unfavourable prognosis. FARSB is an aminoacyl tRNA synthase, and plays a key role in protein synthesis in cells. Furthermore, previous reports have indicated that FARSB is overexpressed in gastric tumor tissues and is associated with a poor prognosis and tumorigenesis. However, the function of FARSB in HCC has not been studied.
Results
The results showed that FARSB mRNA and protein levels were upregulated in HCC and were closely related to many clinicopathological characteristics. Besides, according to multivariate Cox analysis, high FARSB expression was linked with a shorter survival time in HCC and may be an independent prognostic factor. In addition, the methylation level was negatively associated with the expression of FARSB. Furthermore, enrichment analysis showed that FARSB was related to the cell cycle. And TIMER analysis revealed that the FARSB expression was closely linked to tumor purity and immune cell infiltration. The TCGA and ICGC data analysis suggested that FARSB expression is greatly related to m6A modifier related genes. Potential FARSB-related ceRNA regulatory networks were also constructed. What’s more, based on the FARSB-protein interaction network, molecular docking models of FARSB and RPLP1 were constructed. Finally, drug susceptibility testing revealed that FARSB was susceptible to 38 different drugs or small molecules.
Conclusion
FARSB can serve as a prognostic biomarker for HCC and provide clues about immune infiltration, and m6A modification.
Publisher
Research Square Platform LLC
Reference91 articles.
1. Screening for hepatocellular carcinoma in patients with hepatitis C cirrhosis: a cost-utility analysis;ARGUEDAS MR;Am J Gastroenterol,2003
2. ARTERO-CASTRO A, FERNáNDEZ-MARCOS KONDOH,H, SERRANO PJ, RAMóN M Y CAJAL, S. & LLEONART ME. 2009. Rplp1 bypasses replicative senescence and contributes to transformation. Exp Cell Res, 315, 1372-83.
3. ASAFO-AGYEI KO, SAMANT H. 2022. Hepatocellular Carcinoma. StatPearls. Treasure Island (FL): StatPearls Publishing.
4. Copyright © 2022, StatPearls Publishing LLC.
5. N6-methyladenosine demethylase FTO targets pre-mRNAs and regulates alternative splicing and 3'-end processing;BARTOSOVIC M;Nucleic Acids Res,2017