Chromosomal Aberration Analysis: Novel Noninvasive Techniques for Early Stage Cancer Screening A hypothesis for cancer screening

Abstract

Abstract Objective: Chromosome breakage is a catastrophic event that leads to the progressive development and progression of cancer. To analyze the changes of the peripheral blood microenvironment of tumor patients and explore the indicators of non-specific non-invasive tumor early screening and proposed a new idea to explore whether the gene sequence near the break point of DNA damage is the gene sequence that controls the unrestricted growth of normal cells. Methods: The chromosomal aberrations of peripheral blood lymphocytes were analyzed in 60 normal subjects and 49 patients with tumor before radiotherapy. Results: The detection rate of chromosome aberration was high in tumor patients, and "Dicentric + Translocations” of Chromosomes were detected in 36 patients (73.47%). Chi-square test showed statistically significant differences (P <0.01), and chromosome adhesion and dissolution were observed. Conclusions: "Dicentric + Translocation” of chromosome can be used as non-specific early screening indicator for cancer. It is worthy of further study. A new idea to a new concept that FISH (fluorescence in situ hybridization) technique was applied to explore whether the gene sequence near the breakpoint of dicentric chromosome is the gene sequence that controls the unrestricted growth of normal cells, whether this gene controls the unrestricted growth of normal cells to form tumors, using this index to determine the genetic basis of various cancers, at the gene level to modify the base sequence, prevent the occurrence of cancer. It is worthy of further study, and it can provides a new way for gene therapy of tumor.