Genome-wide Mapping of Quantitative Trait Loci Conferring Resistance to Stripe Rust in Spring Wheat Line PI 660072-Reference-Cited by-同舟云学术

Genome-wide Mapping of Quantitative Trait Loci Conferring Resistance to Stripe Rust in Spring Wheat Line PI 660072

Published:2024-01-03 Issue: Volume: Page:
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Author:

Zhou Xinli¹^ORCID,Wang Yuqi²,Luo Yuqi³,Shuai Jie⁴,Jia Guoyun⁴,Chen Hongyang⁴,Zhang Liangqi⁴,Chen Hao⁴,Li Xin⁴,Huang Kebing⁴,Yang Suizhuang⁴,Wang Meinan⁵,Ren Yong⁶,Li Gang⁷,Chen Xianming⁸

Affiliation:

1. Southwest University of Science and Technology

2. Wheat Research Institute,School of Life Science and Engineering,Southwest University of Science and Techonlogy

3. Wheat Research Institute, School of Science and Engineering, Southwest University of Science and Technology

4. Wheat Research Institute,School of Science and Engineering,Southwest University of Science and Technology

5. Department of Plant Pathology,Washington State University

6. Mianyang Institute of Agricultural Science/Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province,Mianyang

7. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University

8. Department of Plant Pathology,Washington State University,USDA-ARS,Wheat Health,Genetics,and Quality Research Unit,Pullman,WA

Abstract

Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Identifying resistance genes is crucial for developing resistant cultivars to control the disease. Spring wheat PI 660072 (Triticum aestivum) has been identified to possess both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust. To elucidate the genetic basis of the resistance in PI 660072, a mapping population consisting of 211 F5 - F7 recombinant inbred lines (RILs) was developed from a cross of PI 660072 with susceptible spring wheat Avocet S. The mapping population was phenotyped for stripe rust responses across five field environments from 2020 to 2022 and genotyped using the 15K SNP (single nucleotide polymorphism) array to map stripe rust resistance loci. The mapping population was also tested at the seedling stage with predominant Chinese Pst races CYR31, CYR32, CYR34 and PST-YX1-3-1 in the greenhouse. Stripe rust resistance genes were identified using the quantitative trait locus (QTL) mapping approach. Two QTL were identified with QYrPI660072.swust-2BL mapped on the long arm of chromosome 2B for ASR and QYrPI660072.swust-4BL on the long arm of chromosome 4B for APR. To facilitate marker-assisted selection breeding, Kompetitive allele specific PCR (KASP) markers, KASP-1269 for QYrPI660072.swust-2BL and KASP-3209 for QYrPI660072.swust-4BL, were developed. These markers could be used to introgress the effective resistance QTL into new wheat cultivars.

Publisher

Research Square Platform LLC

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