Genotyping Investigation of Ciprofloxacin Resistance Genes (Parc And Pare) In Clinical Pseudomonas Aeruginosa Isolates

Abstract

Abstract Purpose Due to Pseudomonas aeruginosa's low susceptibility to the development of antibiotic resistance and antimicrobial drugs, therapy and control of severe infections often result in recurrent difficulties. A bacterial isolate with multidrug resistance (MDR) is one that is resistant to at least one compound from more than three antimicrobial groups, such as aminoglycosides and fluoroquinolones (10). Human burn wounds are complex environments where necrotic tissue and exudates formed from plasma develop. Methods The samples were immediately cultivated on Pseudomonas aeruginosa-selective Blood, MacConkey, Nutrient, and Cetrimide agar media and incubated overnight at 37°C under aerobic conditions. Several biochemical tests, including Oxidase and Catalase tests, were used to identify P. aeruginosa. Additionally, the identification was confirmed using a quick identification procedure (VITEK 2 System). After adding 20% glycerol, the detected isolates were kept at -20°C. Results The findings of the local isolates' sequences being aligned with the reference strain revealed some mismatches in the nucleotides of our query at various locations, including deletions and replacements. According to the findings in table 4, there were discrepancies between the isolates from burn that were resistant to ciprofloxacin (P1 and P16) and the reference bacteria (CP034435). Conclusion The following are the findings of the current study: Pseudomonas aeruginosa is more prevalent in burn samples than in other clinical samples. Misuse of these antibiotics may contribute to an increase in MDR Pseudomonas aeruginosa, particularly resistance to fluroquinolones (Ciprofloxacin).