Abstract
Indoxacarb, an oxadiazine-class of sodium channel blocker insecticide (SCBI), demonstrates remarkable efficacy in combating a diverse array of agricultural pests. Its distinctive neurotoxic mechanism of action disrupts synaptic transmission, presenting a valuable tool for integrated pest management strategies that simultaneously mitigate environmental risks. Brine shrimp (Artemia sp), a zooplanktonic crustacean is one of the most widely occurring euryhaline organisms. It is being extensively used in various bioassay research and applied toxicology experiments, most importantly in the assessment of ecotoxicological impact of xenobiotics. Brine shrimp lethality assay is an important tool for the preliminary cytotoxicity assay of chemicals based on the ability to kill their laboratory cultured larvae (nauplii). The study was conducted to evaluate the in-vivo ecotoxicological effect of indoxacarb, a sodium-channel blocking insecticide (SCBI) on aquatic non-target Artemia species. Insects are the most important target species for indoxacarb. The present toxicological study has estimated an LC 50 value of 5.13 µg/ml with a confidence interval in the range of 0.502 to 0.917 in Artemia sp. The results indicate that indoxacarb possesses a high risk of ecotoxicity to crustaceans when used as an agricultural pesticide.