Affiliation:
1. China Agricultural University
2. Kunming University of Science and Technology
Abstract
Abstract
Eusocial pollinators are crucial elements in global agriculture. The honeybees and bumblebees are associated with a simple yet host-restricted gut community, which protect the hosts against pathogen infections. Recent genome mining has led to the discovery of biosynthesis pathways of bioactive natural products mediating microbe-microbe interactions from the gut microbiota. Here, we investigate the diversity and novelty of biosynthetic gene clusters in the bee gut microbiota by analyzing 477 genomes from cultivated bacteria and metagenome-assembled genomes. We identify 744 biosynthetic gene clusters (BGCs) covering multiple chemical classes. While gene clusters for the post-translationally modified peptides are widely distributed in the bee guts, the distribution of the BGC classes varies significantly in different bee species among geographic locations, which is attributed to the strain-level variation of bee gut members in the chemical repertoire. Interestingly, we find that Gilliamella strains possessing a thiopeptide-like BGC show potent activity against the pathogenic Melissococcus plutonius. The spectrometry-guided genome mining reveals a RiPP-encoding BGC from Gilliamella with a 10 amino acid-long core peptide exhibiting antibacterial potentials. This study illustrates the widespread small-molecule-encoding BGCs in the bee gut symbionts and provides new insight into the bacteria-derived natural products as potential antimicrobial agents against pathogenic infections.
Publisher
Research Square Platform LLC
Reference74 articles.
1. Khalifa SAM, et al. Overview of bee pollination and Its economic value for crop production. Insects 12, (2021).
2. Colony collapse disorder: a descriptive study;Vanengelsdorp D,;PLoS One,2009
3. Evaluation of the distribution and impacts of parasites, pathogens, and pesticides on honey bee (Apis mellifera) populations in East Africa;Muli E,;PLoS One,2014
4. Pathogens, pests, and economics: drivers of honey bee colony declines and losses;Smith KM;Ecohealth,2013
5. Scientific Advances in Controlling Nosema ceranae (Microsporidia) Infections in Honey Bees (Apis mellifera);Burnham AJ;Front Vet Sci,2019