Remyelinating potential of Olea Europaea and Arachis Hypogaea on Experimental autoimmune Encephalomyelitic model of Multiple Sclerosis by downregulating the pro-inflammatory cytokines

Abstract

Multiple sclerosis (MS) is an inflammatory, autoimmune demyelinating central nervous system (CNS) disease. A neuroprotection model is suggested to find novel treatment approaches with oxidative markers interplay in MS. Current study is aimed to find the role of specific neuroinflammatory and oxidative stress biomarkers involved in the progression of EAE-murine-model of MS and to evaluate the neuromodulatory effects of Olea Europaea (Olive oil), and Arachis Hypogaea (peanut oil). Olea Europaea was used for the environmentally friendly synthesis of silver nanoparticles. Marked suppression of leukocyte counts and oxidative-stress markers such as Superoxide dismutase (SOD) (0.66IU/ml), catalase (CAT), (3.89 and 5.56µmol/ml) and Glutathione (GSH) (6.88 µmol/ml) in the cocktail of Olive and Peanut oil extract treated group's serum was noticed. The expression level of interleukin -6 (IL-6) (9.63 ± 0.43) and Tumor necrosis factor- α (TNF-α) was significantly (P≤0.001) increased (7.89±0.24) in the diseased rat group treated with LPS as compared with the control group (1.000±0.00). Olive oil and peanut oil extracts alleviated expression levels of IL-6, TNF-α, INF-α, GAPDH, β-actin and MMP-8. Similarly, standard drµg Nimodipine, Interferon-α, and Dimethyl fumarate also ameliorated pro-inflammatory cytokine production. As per findings, a significant neuroprotective effect with remyelinated axonal-terminal and oligodendrocytes migration, minimal number of lymphocytic infiltrations, and necrosis of Purkinje-cells was observed after treatment with a cocktail of olive and peanut oils by upregulation of Nitric oxide (NO), Matrix metalloprotease-8 (MMP-8) and 8-hydroxy guanosine (8-OHdG) expression levels.