Affiliation:
1. Bahauddin Zakariya University
Abstract
Abstract
The aquaculture industry is dependent on rich fish resources in water bodies. Human activities have led to a rapid decline of fish species. In Asia, the Pangasiidae family is highly valued for its potential for survival and its fillet meat. DNA barcoding is a taxonomic method using genetic markers in organisms mitochondrial DNA (mt DNA) for identification. The phylogeny and identification of Pangasianodon hypophthalmus in the subcontinent is of great concern. For species identification, a precise and rapid technique is DNA barcoding. This method is strongly effective for analyzing the divergence among species. DNA barcoding is more reliable as compared to external morphology. To avoid mislabeling and conservation of species, it is equally useful in juveniles as well as adult stages of fishes. As DNA bar-coding is a taxonomic method that uses small genetic markers in organisms’ mitochondrial DNA (mt DNA) for identification of particular species. In recent study MAGA X and Kimura 2 Parameter was used to evaluate genetic distance and neighbor joining tree was constructed. BOLD and GenBank reveals the nearest identity matches. As mitochondrial cyt-b gene region was successfully used for identifying species and accepted as a standard region for DNA barcoding.
Publisher
Research Square Platform LLC
Reference50 articles.
1. Molecular characterization of Channa species from Bangladesh based on Cytochrome c Oxidase Subunit I (COI) gene;Ahmed SM;FishTaxa,2018
2. DNA Barcoding of Common Commercial Sea Catfish (Genus: Plicofollis) from Kuwait;Al-Zafiri B;Ameri J. of Mol Bio.,2018
3. Applications of DNA barcoding to fish landings: authentication and diversity assessment;Ardura A;Zookeys.,2013
4. DNA barcodes for biosecurity: invasive species identification;Armstrong KF;Philosophical Transactions of the Royal Society B: Biological Sciences,2005
5. Intraspecific phylogeography: The mitochondrial bridge between population genetics and systematics;Avise JC;Annu Rev Ecol Syst.,1987