Abstract
Spotted longbarbel catfish, Hemibagrus guttatus (Lacepède, 1803), is benthic freshwater fish, mainly distributed in southern China, Laos, Thai, and Viet Nam. The wild population of H. guttatus is continuously decreasing, making it essential to protect this valuable fish resources. Understanding the genetic diversity and population structure of H. guttatus is critical to its protection. In the study, we therefore developed 57 InDel markers for H. guttatus by whole genome resequencing. The observed heterozygosity and expected heterozygosity ranged from 0.3333 to 0.5833 and 0.2778 to 0.5000, respectively. The value of polymorphism information content was between 0.2692 and 0.3750. One locus significantly deviated from the Hardy-Weinberg equilibrium (p<0.05). These InDel markers are of great value for genetic diversity and conservation of H. guttatus.