A Novel polysaccharide DSPP-1 from Durian seed: structure characterization and its neuroprotective effects against Alzheimer's disease in a transgenic Caenorhabditis elegans model

Abstract

Abstract Plant polysaccharides have attracted much attention because of their various biological activities. The structure characterization of polysaccharide from durian seed and its neuroprotective effects against Alzheimer's disease (AD) in a transgenic Caenorhabditis elegans model were conducted in this study. A water-soluble polysaccharide was obtained using atmospheric pressure plasma treatment, and named DSPP-1. DSPP-1 was composed of rhamnose, galactose and galacturonic acid and its molecular weight was 3.765×105 Da. The study in vitro showed that 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of DSPP-1 was 79.20% and the inhibitory rate on Aβ1-42 aggregation was 24.65%. In vivo results showed that DSPP-1 could decrease abnormal Aβ1-42 aggregation to delay the paralysis process of AD-nematodes. Moreover, DSPP-1 significantly improved the antioxidant enzyme activities and reduced lipid peroxidation in AD-nematodes. Taken together, these results indicated that DSPP-1 could be used as a potential natural source for the prevention and treatment of AD.