Performance evaluation of Trichoderma reseei in tolerance and biodegradation of diuron herbicide in agar plate, liquid culture and solid-state fermentation

Author:

Reyes-Cervantes Alejandro1,Robles-Morales Diana Laura1,Tec-Caamal Edgar Noe2,Jiménez-González Angélica1,Medina-Moreno Sergio Alejandro1

Affiliation:

1. Universidad Politécnica de Pachuca

2. Tecnologico de Monterrey

Abstract

Abstract

The present work evaluated the performance of the fungus Trichoderma reesei to tolerate and biodegrade the herbicide diuron in its agrochemical presentation in agar plate, liquid culture, and solid-state fermentation. The tolerance to diuron was characterized through a non-competitive inhibition model, showing higher tolerance on the PDA agar plate (Ki=98.63 mg L− 1) than liquid culture with glucose and ammonium nitrate as carbon and nitrogen sources (Ki=39.4 mg L− 1). Diuron biodegradation was characterized through the model of inhibition by the substrate. Trichoderma reesei had a maximum radial growth rate (vrmax) of 1.17 cm d− 1 on agar plate. In liquid culture, Trichoderma reesei biotransformed diuron in 3,4-dichloroaniline using the amide group from the diuron structure as a carbon and nitrogen source, with a maximum specific growth rate (µmax) of 0.0245 h− 1 and a yield of the biomass on the substrate (YXD) of 0.154 mg mg− 1. In solid-state fermentation with a mix of barley straw and agrolite (70:30) as support-substrate, the maximum diuron removal percentage (97.26%) was reached with a specific aeration rate of 1.0 vkgm, inoculum of 2.6x108 spores g− 1, and an initial diuron concentration of 160.81 mg kg− 1. The diuron removal in solid-state fermentation by sorption on the barley straw and agrolite was discarded compared with the removal magnitude of the biosorption and biodegradation mechanisms of Trichoderma reesei. The Trichoderma reesei performance in its tolerance and capability to remove diuron in liquid and solid culture media displays its potential to be used in bioremediation technologies of herbicides polluted sites.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3