Effects of diosgenin dose on alveolar bone loss in rats with experimental periodontitis

Author:

Kızıldağ Alper1,Alpan Aysan Lektemür1,Aydın Tuğba Köseoğlu2,Özdede Melih3,Özmen Özlem4

Affiliation:

1. Pamukkale University

2. Atatürk University

3. Dokuz Eylül University

4. Burdur Mehmet Akif Ersoy University

Abstract

Abstract

Background This study was performed to determine the therapeutic effects of diosgenin (DG) which is a steroidal saponin, administered at different doses on alveolar bone loss (ABL) in rats with experimental periodontitis using immunohistochemical and cone-beam computed tomography (CBCT). Methods Thirty-two male Wistar rats divided into four equal groups: control (non-ligated), periodontitis (P), DG-48, and DG-96. Sutures were placed at the gingival margin of the lower first molars to induce experimental periodontitis. Then, 48 and 96 mg/kg of DG was administered to the study groups by oral gavage for 29 days. At day 30, the animals were sacrificed and ABL was determined via CBCT. Results The expression patterns of osteocalcin (OCN), alkaline phosphatase (ALP), type I collagen (Col-1), B cell lymphoma 2 (Bcl 2), Bcl 2-associated X protein (Bax), bone morphogenetic protein 2 (BMP-2), and receptor activator of NF κB ligand (RANKL) were examined immunohistochemically. DG treatment increased the ALP, OCN, Bcl 2, Col-1, and BMP-2 levels in a dose-dependent manner, compared with the P group (p < 0.05); it decreased the receptor RANKL, Bax, and ABL levels in a dose-dependent manner (p < 0.05). Conclusion DG contributes to bone formation by decreasing ABL and apoptosis in rats with periodontitis.

Publisher

Springer Science and Business Media LLC

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