Abstract
During the last decade the health of honeybee is endangered because of many different pathogens, that is why the isolation of new lactic acid bacteria from honeybees' gut microbiotas, revealing of their strong antibacterial activity will help to create some products for protection of bees’ health.
New Gram-positive mesophilic strains were isolated on selective media for lactic acid bacteria (LAB), followed by species identification by a polyphase approach, including biochemical and physiological characterization and molecular genetic identification based on the sequencing of the 16S rRNA genes. The newly isolated strains were tested for antibacterial activity against a broad range of Gram-positive and Gram-negative species. In total, seventeen new stains possessing antibacterial activity were isolated. They belonged to Bacillus, Staphylococcus, Streptococcus, Lactobacillus and Enterococcus different species. The most promising results comprising the antibacterial activity, and therefore possessing the potential to be used as probiotics, were obtained for the isolates L. kimbladii M9, E. durans M4, S. salivarius M12, L. delbrueckii subs. lactis A4, L. delbrueckii subs. lactis A5, and L. delbrueckii subs. bulgaricus B7. Taken together it can be suggested that strains Enterocccus ratti M2, E. durans M4, L. delbrueckii subs. lactis A4 and L. delbrueckii subs. bulgaricus B7
Mentioned above strains can be applied for protection of honeybees’ health.