Self-assembled AgNCs with United Umbilical Mesenchymal Stem Cell-Derived Exosome-Repair in Spinal Cord Injury Rats

Abstract

Abstract Spinal cord injury (SCI) is a complex process that causes sensory and functional motor deficits, neuronal apoptosis, increased glial scarring, and an inflammatory environment, which is permanent damage. As a therapeutic cell, exosomes can penetrate the blood-brain barrier, regulate inflammation and immune response, inhibit apoptosis, and maintain the blood-spinal cord barrier by promoting angiogenesis and axon outgrowth, promoting the recovery of spinal cord function in rats, is a potential method for the treatment of motor and sensory function in SCI rats, and has attracted much attention in many disease fields. To find a new method for the treatment of spinal cord injury, we choose the Surface enhancement Raman (SERS) and cultured mesenchymal stem cells to obtain exosomes (uMSCEXOs), and then injected them into the injured rats through the tail vein, through observed the damaged parts of the spinal cord of the rats at different time points changes in tissue morphology, changes in inflammatory factors after injury, through the ELISA and SERS detection confirmed the Exos group rats showed high expression of inhibitory inflammatory factor IL-10 and decreased expression of inflammatory factor TNF-α after injury. After damage, the number of neurons in rats treated with exosomes increased. Exosomes will help expand the prospects of umbilical cord mesenchymal stem cells in injury regeneration and repair.