LncRNA018392 promotes proliferation of Liaoning cashmere goat skin fibroblasts through up-regulation of CSF1R by binding to SPI1

Abstract

Abstract The Liaoning cashmere goat has been confirmed as a valuable genetic resource breed that is prohibited from genetic outflow in China, and it achieves the highest single fleece production. Hair follicle development in the cashmere goat is regulated by melatonin and long non-coding RNAs (lnRNAs). However, the role played by lncRNAs in mediating melatonin-promoted cash-mere growth remains unclear. A novel lncRNA-lncRNA018392 with significant overexpression, which played a certain role in the melatonin-promoted proliferation of cashmere skin fibroblasts, was screened in previous research. The flow cytometry and CCK-8 results confirmed that the knockdown of lncRNA018392 reversed the effect of melatonin on cell proliferation, and the prolif-eration of cashmere skin fibroblasts was inhibited after the cells were interfered with the gene CSF1R near lncRNA018392. The dual-luciferase reporter assay further demonstrated that lncRNA018392 can positively regulate the promoter of CSF1R. Moreover, as indicated by the results of RNA-binding protein immunoprecipitation (RIP) and chromatin immunoprecipitation sequencing (ChIP-Seq), lncRNA018392 formed a complex with transcription factor SPI1, and CSF1R served as a downstream target gene regulated by SPI1. As revealed by the results of this study, melatonin-mediated novel lncRNA018392 accelerated the cell cycle, facilitated cell proliferation, and inhibited apoptosis by recruiting SPI1 to up-regulate the expression of nearby gene CSF1R. This study lays a theoretical basis for clarifying the molecular mechanism of cashmere growth and molecular breeding of cashmere goats.