High-throughput sequencing reveals the change of TCR α chain CDR3 with Takayasu arteritis

Abstract

Abstract To research the immunological characteristics of antigen complementarity determining the region of T cell receptor α chain in peripheral blood of patients with Takayasu's arteritis, and to provide the new theoretical basis for the diagnosis and treatment of Takayasu's arteritis. Five untreated patients with Takayasu arteritis in Capital medical university affiliated Anzhen Hospital, China, were collected from June 2019 to December 2019. Four healthy peripheral blood samples were matched as the control group. Separate peripheral blood mononuclear cells and extract RNA for reverse transcription. Specific primers obtained nucleotides in the CDR3 region of the TCR α chain, high-throughput sequencing was performed, and the quality of samples was evaluated by principal component analysis. R software was used for statistical analysis and drawing, and a nonparametric test was used to analyze the differences between the two groups. The results of the principal component analysis showed that the TAK group was in the sample set. D50 analysis showed that the diversity of TCR in the TAK group was significantly higher than in the control group. The expression of 3 genes in the V region was significantly higher in patients than in the control group. 196 VJ rearrangement genes are significantly different between the two groups, of which 47 rearrangement genes in the control group are lower than those in the TAK group, and 149 rearrangement genes in the control group are higher than those in the TAK group. Patients with Takayasu's arteritis have a unique CDR3 library, and there are obvious disease-related T cell clones. These characteristic genes may be a marker for early diagnosis and provide a new theoretical basis for treating Takayasu arteritis.