Affiliation:
1. Beichen District Hospital of Traditional Chinese Medicine, Tianjin University of Chinese Medicine
2. Chu Hsien-I Memorial Hospital, Tianjin Medical University
Abstract
Abstract
It has been reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6A reader IGF2BP1 exhibited significantly higher expression in ccRCC cells. Functionally, results by gainloss functional assay indicated that IGF2BP1 promoted the glucolytic characteristics, including glucose uptake, lactate production and extracellular acidification rate (ECAR). Mechanistically, IGF2BP1 recognizes the m6A modified sites on LDHA mRNA and enhances its mRNA stability, thereby accelerating tumor energy metabolism. Thus, our work reveals a novel facet of the m6A that promotes mRNA stability and highlights the functional importance of IGF2BP1 as m6A readers in post-transcriptional gene regulation.
Publisher
Research Square Platform LLC
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