Affiliation:
1. Laboratory of Experimental Algology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Abstract
The choice of nitrogen source in a cultivation medium can specifically affect the physiology and biochemistry of microalgae. To increase the production of low-cost valuable biomass, the preferred nitrogen form for each alga should be determined. The aim of our study was to analyze the effects of different nitrogen sources and cultivation times on the growth, biochemical composition and the activities of glutamine synthetase, glutamate synthase, glutamate dehydrogenase, malate dehydrogenase, aspartate aminotransferase and proteases of Chlorella vulgaris R-06/2. Media supplemented with urea or ammonium nitrate provided similarly (p>0.05) high growth rates for a short cultivation time (4 days). The two nitrogen compounds applied simultaneously ensured better biomass yield but for prolonged cultivation. In the exponential growth phase, ammonium nitrate stimulated (p<0.05) protein production, whereas urea enhanced (p<0.05) the carbohydrate content in older cultures as compared to the other nitrogen sources. The activity of each of the studied metabolic enzymes of C. vulgaris R-06/2 varied specifically depending on the nitrogen source and the growth phase, ensuring the maintenance of efficient, balanced metabolism under all cultivation conditions. When using large-scale cultivation to produce biomass for various useful applications, the selection of nitrogen source should be based on algal metabolism.
Publisher
National Library of Serbia
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
3 articles.
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