Comparative evaluation of the essential oil terpenoids in the stem and leaf of Ziziphora clinopodioides in the regions of Almeh and Sojough of Golestan province, Iran

Abstract

Microwave-assisted hydrodistillation was used to isolate essential oil from the leaf and stem of Ziziphora clinopodioides collected in the flowering stage on two locations, Almeh and Sojogh, of the Golestan Province (Iran), in June of 2009. The total contents of monoterpene and sesquiterpene fractions (52.45% and 1.08%, respectively) in the leaf oil of Almeh plants were higher than those of Sojogh (46.64% and 0.12%, respectively). The essential oil of the stem of the plants from Sojogh was characterized by the presence of eight oxygenated monoterpenes (22.17%), while four oxygenated monoterpenes (11.15%), one monterpene hydrocarbon (2.71%), and one oxygenated sesquiterpene (0.21%) were found in the plants from the region of Almeh. The analysis of the essential oil of dried aerial parts showed the presence of oxygenated monoterpenes pulegone and menthol (the region of Sojogh) and pulegone, 1,8-cineol, D-neoisomenthol and chrysanthenone (the region of Almeh), as the main constituents. Also, chrysanthenone (9.75%), found as the second major component of the leaf of Z. clinopodioides of Almeh, was not identified as the oil component of the other region. The results obtained on the chemical composition of Z. clinopodioides oil of two regions from the Golestan Province revealed that in general, that there are some differences in the major components and their relative concentrations. This may be probably due to the different environmental and genetic factors, different chemotypes and the nutritional status of the plants, as well as other factors that can influence the oil composition.