Affiliation:
1. Univerzitet u Banjoj Luci, Tehnološki fakultet, Banja Luka, Republika Srpska, Bosna i Hercegovina
2. Univerzitet u Banjoj Luci, Tehnološki fakultet, Vojvode Stepe Stepanovića, Banja Luka, Republika Srpska, Bosna i Hercegovina
3. Univerzitet u Banjoj Luci, Prirodno-matematički fakultet, Banja Luka, Republika Srpska, Bosna i Hercegovina
Abstract
Samples of stinging nettle or common nettle (Urtica dioica L.) were collected
from the area of Banja Luka. To measure and evaluate the content of
chlorophyll (a and b), carotenoids, and soluble proteins, as well as
peroxidase activity (POD, EC 1.11.1.7.), fresh nettle leaves of different
developmental stages were used. Dried nettle leaves were used to obtain
ethanol extract. The dry residue of ethanol extract was dissolved in
methanol and the obtained solution was used to determine the content of
total phenols, flavonoids, flavonols, as well as non-enzymatic antioxidant
activity and antimicrobial activity. The non-enzymatic antioxidant activity
was determined by different methods: FRAP, DPPH, and ABTS. The results were
compared to those of standard substances like vitamin C, BHT, and BHA.
Antimicrobial activity was screened by using macrodilution method. The
obtained results showed insignificantly higher content of chlorophyll,
carotenoids, and proteins in young nettle leaves as well as an increase in
the soluble peroxidase activities. Native electrophoresis of the soluble
fraction showed the presence of two peroxidase isophorms in the soluble
protein fraction of nettle leaves. The total phenolic content in nettle
extracts amounted to 208.37 mg GAE/gdw, the content of total flavonoids was
20.29 mg QE/gdw, and the content of total flavonols was 22.83 mg QE/gdw. The
antioxidant activity determined by FRAP method was 7.50 mM Fe(II)/gdw,
whereas the antioxidant activity measured by using DPPH and ABTS methods,
with IC50 values, were 31.38 and 23.55 ?g mL-1, respectively. These results
showed the weak and moderate antioxidant capacity of stinging nettle.
Extract of Urtica dioica L. was tested for antibacterial acivity against
various Grampositive and Gram-negative bacteria: Bacillus subtilis IP 5832,
Lactobacillus plantarum 299v (Lp299v), Pseudomonas aeruginosa, and
Escherichia coli isolated from food and Escherichia coli isolated from urine
samples. Ampicillin, erythromycin, ciprofloxacin, and gentamicin were used
as positive control. The results showed that minimum inhibitory
concentration (MIC) and minimum bactericidal concentration (MBC) of the
extract ranged from 9.05 to more than 149.93 mg mL-1.
Publisher
National Library of Serbia
Cited by
86 articles.
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