Affiliation:
1. Institute of Molecular Genetics and genetic Engineering (IMGGE), Laboratory for Plant Molecular Biology, Belgrade
Abstract
Mitochondrial genomes (mtDNAs or mitogenomes) of seed plants are
characterized by a notoriously unstable organization on account of which
available so-called universal or consensus primers may fail to fulfil their
foreseen function - amplification of various mtDNA regions in a broad range
of plant taxa. Thus, the primers developed for groups assumed to have similar
organization of their mitogenomes, such as families, may facilitate a broader
usage of more variable non-coding portions of these genomes in group members.
Using in silico PCR method and six available complete mitogenomes of
Fabaceae, it has been demonstrated that only three out of 36 published
universal primer and three Medicago sativa-specific primer pairs that amplify
various mtDNA regions are suitable for six representatives of the Fabaceae
family upon minor modifications, and develop 21 Fabaceae-specific primer
pairs for amplification of all 14 cis-splicing introns in genes of NADH
subunits (nad genes) which represent the most commonly used non-coding mtDNA
regions in various studies in plants. Using the same method and six available
complete mitogenomes of representatives of related families Cucurbitaceae,
Euphorbiaceae and Rosaceae and a model plant, Arabidopsis thaliana, it has
further been demonstrated that applicability of newly developed primer pairs
for amplification of nad introns in more or less related taxa was dependent
not only on species evolutionary distances but also on their genome sizes. A
reported set of 24 primer pairs is a valuable resource which may facilitate a
broader usage of mtDNA variability in future studies at both intra- and
inter-specific levels in Fabaceae, which is the third largest family of
flowering plants rarely studied at the mtDNA level, and in other more or less
related taxa.
Funder
Ministry of Education, Science and Technological Development of the Republic of Serbia
Publisher
National Library of Serbia
Cited by
1 articles.
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