Affiliation:
1. Poljoprivredni institut Osijek, Osijek, Hrvatska
2. Sveučilište u Zagrebu, Agronomski fakultet, Zagreb, Hrvatska
Abstract
Surface sterilization is the most important step in preparation of explants
for micropropagation, because controlling fungal and bacterial contamination
of woody plant from field sources is very difficult. Six sterilizing agents:
sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO)2], sodium
dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl2), silver nitrate
(AgNO3) and hydrogen peroxide (H2O2) were tested for sterilization of
?Oblacinska? sour cherry buds, by varying their concentration and time of
exposure. The aim of this study was to establish best surface sterilization
for in vitro propagation of ?Oblacinska? sour cherry. Aseptic cultures of
?Oblacinska? sour cherry were established from axillary buds which were
placed in nutrient medium, supplemented with plants hormones
6-benzylaminopurine (BA), 1- naphthaleneacetic acid (NAA) and gibberellic
acid (GA3). The results indicated that among these sterilizing agents silver
nitrate (AgNO3) at concentration of 1% for 20 minutes was the best for
controlling the infection, whereas sterilization with sodium
dichloroisocyanurate (DICA) at concentration of 1% for 10 minutes was not
satisfactory.
Publisher
National Library of Serbia
Subject
Plant Science,Soil Science,Agronomy and Crop Science,Animal Science and Zoology
Cited by
47 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献