Affiliation:
1. Institute of Molecular Genetics and Genetic Engineering, Belgrade
Abstract
The recombinant aspartic proteinase-like protein (FeAPL1-His6) was
overexpressed in the tobacco BY-2 cell line and the expected pepstatin
A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry
and protein gel blot analysis of the transformed BY-2 cells and their
protoplasts showed extracellular localization of rFeAPL1-His6 in the cell
wall. Based on the obtained results, potential functions of FeAPL1 are
discussed.
Publisher
National Library of Serbia
Cited by
2 articles.
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