Affiliation:
1. Institute for Animal Husbandry, Belgrade, Zemun, Serbia
2. Laboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
3. Scientific Veterinary Institute Novi Sad, Novi Sad, Serbia
Abstract
The current study aimed to examine the percentage changes of viability sperm
and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm
plasma of Lacaune rams, before and after cryopreservation. For this purpose,
five rams were examined, and two ejaculates were obtained from each ram.
Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou?s sequin method. Sperm viability was determined by
eosin and nigrosine smears, and enzyme activity was examined
spectrophotometrically. As a result, the percentage of vital sperm after
cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing
process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT
/ ALP. In conclusion, the observed enzymes, in relation to sperm vitality,
could be used as indicators to optimize the protocols for cryopreservation
of ram?s sperm.
Publisher
National Library of Serbia
Reference34 articles.
1. ALAMAARY M.S., HARON A.W., HIEW M.V.H., ALI. M. (2020): Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post-thawed stallion semen. Veterinary Medicine and Science, 6, 4, 666-672.
2. ANDREEVA M. (2020): Investigation of the effect of breed characteristics in sheep on cryotolerance of sperm. Ph.D. Dissertation. Institute of Biology and Immunology of Reproduction „Akad.Kiril Bratanov”, Bulgarian Academy of Sciences, Sofia, (in Bulgarian).
3. ANDREEVA M., KARADJOVA V., STEFANOV R. (2022): Investigation of the effect of ultra-low temperatures in cryopreservation on the activity of the enzymes lactate dehydrogenase and gamma-glutamyl transferase. Journal of Chemical Technology and Metallurgy, 57, 2, 298-301.
4. ANDREEVA M., STEFANOV R. (2020a): Influence of the cryopreservation on the vitality of the sperm of the different breeds of rams. Tradition and Modernity in Veterinary Medicine, 5, 2, 9, 26-30. doi: 10.5281/zenodo.4317364
5. ANDREEVA M., STEFANOV R. (2020b): Study of the relationship between the age of the rams and the quality of their ejaculates obtained outside the breeding season. Biotechnology in Animal Husbandry 36, 4, 437-445. doi: 10.2298/BAH2004437A