The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation-Reference-Cited by-同舟云学术

The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation

Published:2010 Issue:3 Volume:62 Page:833-840
ISSN:0354-4664
Container-title:Archives of Biological Sciences
language:en
Short-container-title:ARCH BIOL SCI BELGRA

Author:

Nikolic D.¹,Djordjevic P.B.¹,Dimitrijevic-Sreckovic Vesna¹,Paunovic I.¹,Kalezic Nevena¹,Popovic S.¹,Stefanovic D.²,Dzingalasevic Marina³

Affiliation:

1. Faculty of Medicine, Belgrade + Clinical Centre of Serbia, Institute for Endocrinology, Diabetes and Metabolic Diseases, Laboratory for Pancreatic Islet Culture, Belgrade

2. Faculty of Medicine, Belgrade + Clinical Centre of Serbia, Surgical Clinic, Institute of Digestive Diseases, Belgrade

3. Clinical Centre of Serbia, Institute for Endocrinology, Diabetes and Metabolic Diseases, Laboratory for Pancreatic Islet Culture, Belgrade

Abstract

Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets. The aim of this study was to investigate the effect of different concentrations of Liberase HI (1.5, 2.5 and 3.5 mg/ml) on the yield, viability and function of islets isolated by a non-automated method. Based on our results, the Liberase HI concentration 1.5 mg/ml is recommended for this method of isolation. .

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

Reference6 articles.

1. SIGNIFICANT PROGRESS IN PORCINE ISLET MASS ISOLATION UTILIZING LIBERASE HI FOR ENZYMATIC LOW-TEMPERATURE PANCREAS DIGESTION1

2. Successful Human Islet Isolation Utilizing Recombinant Collagenase

3. Apoptosis of human fetal pancreatic islets during short-term culture

4. Collagenases

5. Evaluation of a Purified Enzyme Blend for the Recovery and Function of Canine Pancreatic Islets

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