Affiliation:
1. R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist: Dhule (M.S.) India
Abstract
A simple, precise, accurate, sensitive and repeatable RP-UPLC method was
developed for quantitative determination of atropine sulphate in
pharmaceutical dosage form. The method was developed by using C18 column
Hiber HR Purospher Star (100mm?2.1mm id, 2?m particle size) as stationary
phase with Phosphate Buffer: Acetonitrile (87:13, %v/v) as a mobile phase, pH
was adjusted to 3.5 by ortho-phosphoric acid at a flow rate of 0.5 mL/min and
column temperature maintained at 30?C. Quantification of eluted compound was
achieved with PDA detector at 210 nm. Atropine sulphate followed linearity in
concentration range of 2.5-17.5 ?g/mL with r2=0.9998 (n=6). Limit of
detection (LOD) and limit of quantification (LOQ) values were 0.0033 and
0.0102 ?g/mL for atropine sulphate. The validation study is carried out as
per International Conference on Harmonization (ICH) guidelines. This method
was successfully applied for estimation of atropine sulphate in
pharmaceutical formulation.
Publisher
National Library of Serbia
Subject
General Chemical Engineering
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献