Affiliation:
1. Faculty of Science and Mathematics, Department of Chemistry, Niš
2. Faculty of Chemistry, Belgrade
Abstract
Propranolol, a widely used beta-blocker, inhibits the reaction of enzyme
cholinesterase hydrolysis. Measurements of the hydrolysis rate differences
between and inhibited reactions, allows the development of a kinetic method
for its determination. Both systems, enzyme-substrate-chromogen and
enzyme-substrate-chromogen-inhibitor, were characterized through biochemical
kinetic parameters (KM = 0.326-0.330 mmol/L; Vmax = 40-42.99 ?mol/Lmin),
inhibition type was recognized as competitive, and inhibition constant, Ki,
was determined to be 22.60 ?mol/L. The detection and quantification limits
were calculated as 0.004 and 0.0136 ?mol/L, respectively. Accuracy and
precision of proposed methods were tested. Proposed method is characterized
with good sensitivity, selectivity, simplicity and rapidity, thus it is
convenient for clinical applications.
Publisher
National Library of Serbia
Subject
General Chemical Engineering,General Chemistry
Cited by
3 articles.
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