Affiliation:
1. Faculty of Technology, Leskovac
Abstract
In vitro chlorophyll a degradation by horseradish peroxidase in the presence
of the resorcinol was investigated in this work, and the influence of pH and
temperature was particularly studied. Chlorophyll a degradation was followed
by UV-VIS and HPLC. Chlorophyll a was degraded when hydrogen peroxide was
added into reaction mixture containing chlorophyll fraction, horseradish
peroxidase, resorcinol and phosphate buffer. HPLC analysis has identified the
main degradation product of chlorophyll a as 132 -hydroxychlorophyll a. The
degradation was traced at different temperatures and pH values. The
increasing temperatures led to increase of chlorophyll a degradation, with a
maximum at 37 ?C. The degradation also increased with increasing pH values,
reaching maximum at pH 6.
Publisher
National Library of Serbia
Subject
General Chemical Engineering,General Chemistry
Cited by
8 articles.
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