Affiliation:
1. Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu, India
Abstract
Optimization of the growth condition for maximum growth rate and protease
production was carried out using Bacillus subtilis. The optimization of
protease production using agro industrial waste product such as cassava waste
as substrate was performed with statistical methodology based on experimental
designs. The screening of twelve nutrients for their influence on protease
production was achieved using a Plackett-Burman design. MgSO4.7H2O, casein
and glucose were selected based on their positive influence on protease
production. The selected components were optimized using Response Surface
Methodology (RSM). The optimum conditions are (% w/w): MgsO4.7H2O- 0.14,
casein- 1.4 and glucose- 2.64. These conditions were validated experimentally
which revealed an enhanced protease yield of 202.048 U/gds.
Publisher
National Library of Serbia
Subject
General Chemical Engineering
Cited by
5 articles.
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