Solanum pseudo-capsicum effects on Bax and Bcl-2 gene expression and apoptosis in MCF-7 cell line

Abstract

In the present study, the effect of Solanum pseudo-capsicum extract on MCF-7 cell line was investigated. Control cell line in this study was HFF skin fibroblast cell line. Cells have been exposed to the treatment for 24h, 48h and 72 hours respectively. Then, the viability of dual cell lines was assessed using MTT assay. Its results showed that 72-hour treatment with plant extract at IC50 concentration had the greatest effect on cancer cell death. Flow cytometry results showed that 48 hours of treatment with plant extract at IC50 concentration, the highest rate of induced apoptosis occurs. Then, Real time PCR test was performed to measure changes in the expression of Bax and Bcl-2 genes The reference gene in this study was ?-actin. The results of this study showed that in cancer cells treated with the plant extract, the expression of Bax pro-apoptotic gene increased and the expression of anti-apoptotic gene Bcl-2 decreased. Therefore, 72-hour treatment had the greatest effect on increasing Bax gene expression and decreasing Bcl-2 gene expression. Due to the lower side effects of plants compared to conventional chemical drugs used in the treatment of cancer, it is possible to take an effective step in the treatment of this disease by using new methods in identifying effective plant compounds and purifying them.