Genetic variants of endothelial nitric oxide synthase (eNOS 4a/b) and susceptibility to renal cell carcinoma in a Turkish population
Author:
Ceylan Gozde1, Gumus Nur1, Seckin Sule1, Sanli Oner2, Erdem Selcuk2, Kucukgergin Canan1
Affiliation:
1. Department of Medical Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey 2. Department of Urology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey
Abstract
Nitric oxide (NO), plays a significant part in biological processes.
Endothelial nitric oxide synthase (eNOS), the enzyme that catalyzes the
generation of NO in endothelium, contains genetic polymorphisms that have
been linked to an increased risk of developing cancer. The purpose of this
investigation was to ascertain whether there is any connection between renal
cell carcinom (RCC) and the eNOS 4a/b gene polymorphism. This study included
94 patients (mean age:54.2?10.5 years) diagnosed with histopathologically
confirmed RCC and 188 healthy controls (mean age:56.7?11.1 years). eNOS 4a/b
gene polymorphism was examined in DNA samples taken from patients and
healthy controls using polymerase chain reaction (PCR) and agarose gel
electrophoresis methods. The patient and control groups did not differ
statistically significantly in terms of age or body mass index. The most
frequent genotype of eNOS 4a/b gene polymorphism is bb genotype in a Turkish
population. When compared to the control group, patients with RCC had
significantly higher rates of the aa and ab genotypes (p= .018 and p= .000,
respectively). There was no discernible difference in the eNOS 4a/b gene
polymorphism between patients with high-grade and advanced-stage disease and
those with low-grade and stage disease. We suggest that the eNOS 4a/b gene
polymorphism may be effective in the onset of renal cell cancer, but it is
not effective in development.
Publisher
National Library of Serbia
Subject
Plant Science,Genetics
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