Mucoid bovine mastitis isolates of Streptococcus uberis - difficulties in identification-Reference-Cited by-同舟云学术

Mucoid bovine mastitis isolates of Streptococcus uberis - difficulties in identification

Published:2022 Issue:1 Volume:76 Page:85-90
ISSN:0350-2457
Container-title:Veterinarski glasnik
language:en
Short-container-title:VET GLASNIK

Author:

Stevanovic Oliver¹,Milanov Dubravka²^ORCID,Djilas Milan³,Aleksic Nevenka⁴^ORCID

Affiliation:

1. Dr Vaso Butozan Veterinary Institute of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina

2. Novi Sad Scientific Veterinary Institute, Novi Sad, Serbia

3. Institute of Public Health of Vojvodina, Novi Sad, Serbia

4. Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

Abstract

Highly mucoid Streptococcus uberis were isolated on blood agar from five milk samples originating from two dairy cow farms. All the isolates were CAMP test and esculin hydrolysis negative. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) following prior extraction with 70% formic acid was used for identification to the species level. Given that it is plausible that such strains could be isolated in the future from dairy cows by laboratories in Serbia and the Balkans, we consider this case report to be a useful instruction for veterinary bacteriology laboratories.

Funder

Ministry of Education, Science and Technological Development of the Republic of Serbia

Publisher

National Library of Serbia

Reference13 articles.

1. Bradley A. J. 2002. Bovine mastitis: An evolving disease. The Veterinary Journal, 164: 116-128.

2. EUCAST, 2021. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0, 2021. http://www.eucast.org

3. Fortin M., Messier S., Julie Pare J., Higgins R. 2003. Identification of catalase-negative, non-beta-hemolytic, Gram-positive cocci isolated from milk samples. Journal of Clinical Microbiology, 41:106-109. DOI: 10.1128/JCM.41.1.106-109.

4. Günther J., Czabanska A., Bauer I., Leigh J. A., Otto Holst O., HansMartin Seyfert H-M. 2016. Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages. Veterinary Research 47:13. DOI 10.1186/s13567-015-0287-8.

5. Khan I. U., Hassan A. A., Abdulmawjood A., Lämmler C., Wolter W., Zschöck M. 2003. Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods. Journal of Veterinary Science, 4:213-223. PMID: 14685025.