Affiliation:
1. College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei Province, China
2. College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, Hubei Province, China + College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hubei Province, China
Abstract
Rhododendron fortunei (Ericaceae) possesses valuable horticultural and
medicinal values. However, the genomic information on R. fortunei is very
limited. In this study, the complete chloroplast genome (cp) of R. fortunei
was assembled and annotated, SSR loci were characterised, comparative
genomic analysis was carried out, and phylogenetic research was also
performed. The results showed that the R. fortunei cp genome was of a
typical quadripartite structure (200,997 bp). The lengths of the large
single copy region (LSC), the inverted repeat regions (IR), and the small
single copy region (SSC) were 109,151 bp, 2,604 bp, and 44,619 bp,
respectively. A total of 147 unique genes were identified, including 99
protein-coding genes, 42 tRNA genes, and 6 rRNA genes, respectively. Leucine
(11.51%) and cysteine (1.15%) were the highest and lowest representative
amino acids, respectively. The total of 30 codons with obvious codon usage
bias were all A/U-ending codons. Among the 77 simple sequence repeats, the
majority were mononucleotide A/T repeats located in the intergenic spacer
region. Five gene regions showed high levels of nucleotide diversity (Pi >
0.03). The comparative genome analysis revealed 7 hotspot intergenic regions
(trnI-rpoB, trnTrpl16, rpoA-psbJ, rps7-rrn16, ndhI-rps16, rps16-rps19, and
rrn16-trnI), showing great potential as molecular makers for species
authentication. Expansion and contraction were detected in the IR region of
the R. fortunei cp genome. In the phylogenetic tree, R. fortunei was closely
related to R. platypodum. This research will be beneficial for evolutionary
and genetic diversity studies of R. fortunei and related species among the
Ericaceae family.
Publisher
National Library of Serbia
Cited by
1 articles.
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