Affiliation:
1. Department of Molecular Biology and Genetics, Faculty of Science and Literature, Afyon Kocatepe University, Afyonkarahisar, Turkey
Abstract
Soil salinization is an important environmental problem affecting
agricultural production worldwide. Seed germination is a critical process,
and seedling establishment under saline conditions can be achieved by
successful germination. In the present study, comparative proteomics
combined with physiological analyses were used to investigate the protein
alterations in germinating Brassica napus cultivars (Caravel and Sary) under
NaCl stress. Seed germination declined with the increasing NaCl
concentration. However, Caravel exhibited better performance in terms of
seed germination and seedling growth under salinity stress. Therefore,
Caravel was found to be more tolerant to salinity than Sary. The root
proteins were extracted from B. napus cultivars germinating on a plant
growth medium with or without 100 mM NaCl for seven days. After the root
proteins had been separated by two-dimensional (2-D) gel electrophoresis,
the differentially accumulated proteins were identified using MALDI-TOF/TOF
MS. The comparative proteomics analysis revealed 12 and 27 statistically
significant proteins accumulated in the NaCl-treated roots of Caravel and
Sary, respectively. The identified proteins were mostly involved in protein
metabolism, stress defense, cytoskeleton and cell wall metabolism, and
energy metabolism. The salt-sensitive cultivar Sary displayed an elevated
accumulation of proteins involved in antioxidant defense and the protein
catabolic process such as superoxide dismutase [Fe], L-ascorbate peroxidase
1, and different components of the proteasome system. On the other hand, the
levels of molecular chaperones including 20 kDa chaperonin, chaperonin
CPN60, heat shock cognate protein HSC70, and heat shock 70 kDa protein 1
were higher in Caravel than Sary under salt stress. These findings will
provide the possible mechanisms which contribute to salt tolerance and may
serve as the basis for improving salinity tolerance in rapeseed.
Publisher
National Library of Serbia
Cited by
1 articles.
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