Integration of miRNA-lncRNA-mRNA profiles in liver tissue from EpCAM knockout mice-Reference-Cited by-同舟云学术

Integration of miRNA-lncRNA-mRNA profiles in liver tissue from EpCAM knockout mice

Published:2022 Issue:1 Volume:74 Page:25-39
ISSN:0354-4664
Container-title:Archives of Biological Sciences
language:en
Short-container-title:ARCH BIOL SCI BELGRA

Author:

Lei Zili¹,Lei Yuting¹,Chen Guibin¹,Liu Shaomin²,Liu Wanwan¹,Huang Li²,Yang Lanxiang²,Wu Huijuan²,Yang Yanhong³

Affiliation:

1. Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China

2. Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine; Guangdong TCM Key Laboratory for Metabolic Diseases; Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China; Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. China + School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P.R. China

3. First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Guangzhou, P.R. China

Abstract

The epithelial cell adhesion molecule (EpCAM) is highly expressed in the liver during development and diseases. However, its role in the development and pathology of liver remains to be explored. The liver tissues of EpCAM-/- and wildtype (WT) mice at P0 stage were used for RNA sequencing. The differently expressed miRNAs, lncRNAs and mRNAs were selected and confirmed by qPCR. The expression of metabolism-related gene SET domain bifurcated 2 (Setdb2) was significantly increased in the liver of EpCAM-/- mice; the triglyceride (TG) and total cholesterol (TC) levels in the liver were also markedly decreased in EpCAM-/- mice. The microRNA (miRNA)-long noncoding RNA (lncRNA)-mRNA regulatory networks indicated that EpCAM may play important roles in glucose and lipid metabolism of the liver during development and in disease. The comprehensive miRNA, lncRNA and mRNA expression profiles in the developing liver of EpCAM-/- mice established here might help to elucidate functions and mechanisms of EpCAM during development and in diseases of the liver.

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

Reference42 articles.

1. Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, Yashiro K, Tsukita S, Hamada H. EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins. Dev Biol. 2012;371(2):136-45. https://doi.org/10.1016/j.ydbio.2012.07.005

2. Huang L, Yang Y, Yang F, Liu S, Zhu Z, Lei Z, Guo J. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018;42(4):1771-85. https://doi.org/10.3892/ijmm.2018.3764

3. Das B, Okamoto K, Rabalais J, Marchelletta RR, Barrett KE, Das S, Niwa M, Sivagnanam M. Congenital tufting enteropathy- associated mutant of epithelial cell adhesion molecule activates the unfolded protein response in a murine model of the disease. Cells. 2020;9(4):946. https://doi.org/10.3390/cells9040946

4. Das B, Okamoto K, Rabalais J, Kozan PA, Marchelletta RR, McGeough MD, Durali N, Go M, Barrett KE, Das S, Sivagnanam M. Enteroids expressing a disease-associated mutant of EpCAM are a model for congenital tufting enteropathy. Am J Physiol Gastrointest Liver Physiol. 2019;317(5):G580- 91. https://doi.org/10.1152/ajpgi.00098.2019

5. Jiang L, Shen Y, Guo D, Yang D, Liu J, Fei X, Yang Y, Zhang B, Lin Z, Yang F, Wang X, Wang K, Wang J, Cai Z. EpCAMdependent extracellular vesicles from intestinal epithelial cells maintain intestinal tract immune balance. Nat Commun. 2016;7:13045. https://doi.org/10.1038/ncomms13045