Marginal microleakage of newly synthesized nanostructured materials based on calcium aluminate after application in interradicular perforations

Abstract

Introduction. Marginal seal or adequate marginal adaptation of material along the cavity walls should be able to prevent leakage of tissue fluid and consequently bacterial microleakage, terefore, it is considered as significant factor for long term success of endodontic treatment. The aim of this work was to test the marginal microleakage of newly synthesized nanostructured biomaterials based on calcium aluminate, calcium silicate and MTA with a dye penetration test after application the material to the interradicular perforations of extracted teeth. Material and method. The study included 48 extracted human maxillary and mandibular molars. Newly synthesized nanostructured materials were tested: material based on calcium aluminate, calcium silicate. Commercial calcium silicate cement (MTA Angelus, Londrina, Brazil) was used as a control material. Marginal microleakage was examined with a dye penetration test six months after application of the material in experimentally prepared interradicular perforations on extracted human molars. Penetration depth measurement was analyzed with a binocular magnifier (Leica DM 500, Leica Byosistems). Results are expressed in millimeters and statistically processed by applying the analysis of variance for repeated measurements with the Sidak test. Results. The lowest average penetration (mm) was for MTA (1.40 ? 0.63 mm), and the highest for Ca aluminates (2.10 ? 0.63 mm), while for calcium silicates an average color penetration of 1.73 ? 0, 67mm was recorded. By testing intergroup differences in color penetration between groups, a statistically significant difference between MTA and Ca aluminate was obtained using Sidak?s test (t = -0.693; p = 0.036). No statistically significant difference was recorded between MTA and Ca silicate, nor was there a statistically significant difference between Ca aluminate and Ca silicate. Conclusion. The lowest marginal microleakage, the best marginal sealing, was recorded with the MTA material. The microleakage of calcium aluminate-based materials was significantly higher compared to calcium silicate and MTA.