The influence of molecular weight of polyethylene glycol on separation and purification of pectinases from Penicillium cyclopium in aqueous two-phase system

Abstract

In this study the possibility of the partitioning and purification of pectinases from Penicillium cyclopium by their partitioning in polymer/polymer and polymer/salt aqueous two-phase systems was investigated. In the system with 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500 000/85% (w/w) crude enzyme, the highest values for partitioning parameters were achieved - the partition coefficient was 2.11, followed by the top phase yield of 85.68% and purification factor 1.28 for the endo-pectinase activity. The partition coefficient, yield in the top phase and purification factor for the exo-pectinase activity in the same system were 1.89, 84.28% and 3.82, respectively. In the system with 10% (w/w) polyethylene glycol 6000/15% (w/w) (NH4)2SO4/75% (w/w) crude enzyme purification factor 37.85 for exo-pectinase, and 19.52 for endo-pectinase in the bottom phase were obtained.