Genetic diversity and relationships among salvia species by ISSR markers

Author:

Yanpeng Zhao1,Hongmei Wang2,Wei Liang3,Khayatnezhad Majid4,Faisal F5

Affiliation:

1. Department of Fundamental Courses, Shandong University of Science and Technology, Taian, Shandong, China

2. Dept.of Physical Education, University of Science and Technology Liaoning, Anshan, China

3. Institute of Physical Education,Qingdao University of Science and Technology,Qingdao Shandong, China

4. Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran

5. Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan

Abstract

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

Publisher

National Library of Serbia

Subject

Plant Science,Genetics

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