Affiliation:
1. Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
2. Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Abstract
Infertility is a major health human problem with 10% to 15% of world-wide
occurrence. It may happen due to various reasons including physiological,
environmental, social, and genetic factors. Recent genetic investigations
show that hundreds of genes may act on infertility, each with minor effects.
Asthenozoospermia is one of the common types of infertility in men which is
determined by sperm motility reduction. CRISP2 gene is located on acrosome
and tail of sperm and plays crucial role in spermatogenesis, regulation of
sperm flagellum motility, acrosome reaction and gamete fusion. In general,
heterogeneous results are present for association of CRISP2 gene with
asthenoteratozoospermic male infertility, therefore, we carried out a
starting investigation to show association of rs1294845152 of CRISP2 Gene
with male infertility by RFLP analysis. We also tried to identify the known
SNPs and variant nucleotides adjacent to this SNP by sequencing. The present
study revealed that neighther rs1294845152, nor the neighboring sequences
show any association with this kind of male infertility in the limited
samples studied in Iran. In general, it seems that this part of men genetic
material shows a high degree of conservation and very low level of
nucleotide variability.
Publisher
National Library of Serbia