Affiliation:
1. Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract
Persian hogweed (Heracleum persicum Desf. ex Fischer) is native to Iran but
was introduced to Europe as an invasive plant. It has medicinal and edible
values. Its fruits have been used to relieve flatulence, stomach aches as
well as a flavoring as a digestive and an antiseptic. This medicinal plant
forms many geographical populations in the country, particularly in
mountainous regions; however, we have no data on the genetic structure and
genetic diversity of this plant species. Such information can be used in
gene pool identification and future genetic conservation and breeding
programs. Therefore, the present study was performed with the following
aims: 1- Provide data on genetic diversity of geographical populations, 2-
Identify the potential gene pools for future genetic conservation and
breeding programs. We used both Inter-simple sequence repeats (ISSR) and the
Start Codon Targeted (SCoT) molecular markers as these are suitable
molecular markers for genetic diversity investigations. There are highly
polymorphic, reproducible, and relatively low cost markers. The present
study revealed that both ISSR and SCoT markers produce data on the genetic
variability and genetic affinity of the local populations. Both molecular
markers revealed a good level of genetic variability within and among
Heracleum persicum populations. Analysis of molecular variance (AMOVA)
produced a significant differences between geographical populations for both
markers. We could identify few ISSR as well as SCoT bands which can
differentiate the studied populations. The moderate to a good level of
genetic diversity we observed within each H. persicum may be due to cross
pollination. In conclusion, we suggest using combination of ISSR and SCoT
molecular markers to study population genetic variability in H. persicum
geographical populations for future genetic conservation and germplasm
collection of this medicinal plant.
Publisher
National Library of Serbia
Cited by
1 articles.
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