Immunohistochemical expression of p53 oncoprotein in Wilms tumour in relation to histological components, histological types and preoperative chemotherapy

Author:

Djuricic Slavisa1,Djokic Dragomir1,Vujic Dragana1,Basta-Jovanovic Gordana2,Todorovic Vera3,Radojevic-Skodric Sanja2,Zdravkovic Slavisa1,Vujanic Gordan4

Affiliation:

1. Mother and Child Health Institute of Serbia “Dr Vukan Čupić”, Belgrade

2. School of Medicine, Institute of Pathology, Belgrade

3. Institute for Medical Research, Belgrade

4. Cardiff University, School of Medicine, Department of Pathology, Cardiff, UK

Abstract

INTRODUCTION. There have been only few studies of immunoexpression of p53 in Wilms tumour (WT), and their results are somewhat contradictory. OBJECTIVE. The aim of the study was to determine p53 immunohistochemical expression in WT in relation to its histological components, histological prognostic types classified according to the SIOP Working Classification of Renal Tumours of Childhood (2001), and influence of preoperative chemotherapy. METHOD. The analyses are based on 79 primary WTs treated in single institution according to SIOP protocols between 1983-2001. For the immunohistochemical detection of p53, the monoclonal p53 antibody (DO-7, DAKO) was used. Semiquantitative grading of nuclear staining was done. RESULTS. The immunoexpression of p53 was significantly higher in the blastemal and epithelial than in the stromal component (p<0.001). It was significantly correlated to WT histological prognostic types (p=0.039). The exensivity of p53 immunoexpression was higher in anaplastic components but a difference between WT type of diffuse anaplasia and all other types was nonsignificant (p=0.10). Five blastemal type WTs were p53 immunopositive and four immunonegative. There was no difference in p53 immunopositivity between WT treated with the preoperative chemotherapy and primary resected WT (p=0.88). CONCLUSION. The immunoexpression of p53 in WT was significantly higher in the blastemal and epithelial than in the stromal component. It was in significant correlation with histological types of WT. The anaplastic component had noticeable but statistically not significantly higher p53 immunoexpression than non-anaplastic. The preoperative chemotherapy did not modify p53 immunoexpression of WT which had been found in other similar studies.

Publisher

National Library of Serbia

Subject

General Medicine

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