Affiliation:
1. Trakia University, Faculty of Agriculture, Department of Genetics, Animal Breeding and Reproduction, Stara Zagora, Bulgaria
2. Executive Agency of Animal Breeding and Reproduction, Sofia
3. AgroBioInstitute, Sofia, Bulgaria
Abstract
The aim of the study is focused on the design of a conservation sheep
breeding programme for the studied autochthonous breeds based on SSR markers
genetic variation analysis applied. Seven local Bulgarian autochthonous sheep
breeds (Breznishka, Sofiiska, Copper-Red Shumenska, Karakachanska, Local
Karnobatska, Blackhead Plevenska and Starozagorska) were differentiated using
six microsatellite (SSR) markers, aiming to assess the genetic variation
within and between breeds. Among the total of 96 identified alleles, eighteen
population specific ones were detected across the all studied genotypes of
338 individuals, except in the Blackhead Plevenska sheep. All examined breeds
indicated high level of genetic diversity, with an average of 0.792. The
genetic differentiation between the examined sheep breeds was not significant
and the values of genetic distances were relatively low. The analysis of
molecular variances (AMOVA) showed low variation between the examined breeds
(5.51%) in comparison to within population variation (94.49%). The greatest
distance (0.643) was found between the populations Local Karnobatska and
Starozagorska, while the smallest one (0.108), between the Copper-Red
Shumenska and Karakachanska. The genetic distances calculated by
Neighbour-Joining method, produced a phylogenetic tree which separates the
investigated sheep breeds into two main clusters: one including Blackhead
Plevenska Breznishka and Local Karnobatska, and the other one consisting of
the four remaining breeds - Copper-Red Shumenska, Karakachanska, Sofiiska and
Starozagorska sheep. The Factorial Correspondence Analysis (FCA) showed
isolation of Local Karnobatska sheep and Starozagorska and an admixture of
the other populations.
Publisher
National Library of Serbia
Cited by
5 articles.
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