Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae-Reference-Cited by-同舟云学术

Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

Published:2009 Issue:4 Volume:61 Page:713-718
ISSN:0354-4664
Container-title:Archives of Biological Sciences
language:en
Short-container-title:ARCH BIOL SCI BELGRA

Author:

Loncar N.¹,Bozic Natasa²,Nenadovic Vera³,Ivanovic Jelisaveta³,Vujcic Z.¹

Affiliation:

1. Katedra za biohemiju, Hemijski fakultet, Beograd

2. Centar za hemiju, Institut za hemiju, tehnologiju i metalurgiju, Beograd

3. Odeljenje za fiziologiju i biohemiju insekata, Institut za biološka istraživanja 'Siniša Stanković', Beograd

Abstract

The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.

Publisher

National Library of Serbia

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

Reference13 articles.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

2. Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

3. Detection and quantification of leucyl aminopeptidase after native electrophoresis using leucine-p-nitroanilide

4. Partial purification and characterization of midgut leucyl aminopeptidase of Morimus funereus (Coleoptera: Cerambycidae) larvae

5. Partial purification and characterization of the major midgut proteases of grass grub larvae (Costelytra zealandica, Coleoptera: Scarabaeidae)

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