Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules-Reference-Cited by-同舟云学术

Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

Published:2008 Issue:1 Volume:6 Page:105-117
ISSN:0354-4656
Container-title:Facta universitatis - series: Physics, Chemistry and Technology
language:en
Short-container-title:Facta Univ Phys Chem Technol

Author:

Terzic M.¹,Marinkovic B.P.²,Sevic D.²,Jureta J.³,Milosavljevic A.R.³

Affiliation:

1. Institute of Physics, Belgrade + Faculty of Sciences, Novi Sad

2. Institute of Physics, Belgrade + The Advanced School of Electrical Engineering and Computing, Belgrade

3. Institute of Physics, Belgrade

Abstract

Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules.

Publisher

National Library of Serbia

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