Acid and enyzymatic hyrolysis of Solarium tuberosum L. Glycoalkaloids: Comparison results

Abstract

A comparison of the results of the acid and enzymatic hydrolysis of GA from potato haulm and tuber sprouts is presented in this paper. Different systems were used for acid hydrolysis: monophase liquid, two-phase liquid-liquid or solid-liquid and three-phase solid-liquid-liquid systems. Enzymatic hydrolysis was carried out by enzymes present in fresh haulm and fresh tuber sprouts and the fresh juice obtained from fresh plant material. The procedure of acid hydrolysis of GA from potato sprouts in a two-phase liquid-liquid system was selected as the optimal procedure for obtaining solanidine. The liquid-liquid system was composed of GA extract with hydrochloric acid as the first liquid phase and chloroform as the second liquid phase. A degree of GA hydrolysis of 96% and a solanidine yield of 1.50 g per 100 g of dried tuber sprouts after 90 minutes were then achieved.