Redox status of pregnant women with thrombophilia

Abstract

Background/Aim. Considering the fact that role of oxidative stress in pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of our study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women who were divided into two groups: thrombophilia and normal pregnancy group. The thrombophilia group consisted of 60 pregnant women with thrombophilia, while the normal pregnancy group included 60 physiologically healthy pregnant women. Blood samples for biochemical analysis were collected at the end of first, second and third trimester of pregnancy. Concentrations of hydrogen peroxide (H2O2), nitrites (NO2-) and index of lipid peroxidation measured as TBARS were measured in plasma. Level of reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT) were measured in erythrocytes. Results. In women with thrombophilia, NO2- values were increased in the first and third trimester when compared with healthy pregnant women (p<0.05). The higher levels of TBARS and H2O2 were noticed in women with thrombophilia in the first trimester when compared to healthy pregnant women (p<0.05). The values of SOD and CAT were lower in women with thrombophilia in the third and GSH in the first trimester compared to control group (p<0.05). Conclusion. Our results suggest increased generation of pro-oxidants in thrombophillia at the beginning of gestation, which declines as gestation progresses and reaches the similar values as in normal pregnancy at the end of pregnancy. Generally viewed, thrombophilia was associated with impaired antioxidant capacity - SOD and CAT were lower in the third and GSH in the first trimester compared to healthy women.