Affiliation:
1. School of Intelligent Construction, Luzhou vocational and technical college, Luzhou, Sichuan, China
Abstract
Stellaria L. (Caryophyllaceae, Alsinoideae) comprises ca. 150-200 species
across the world. Stellaria species are common herbs, preferred humid
mountainously slopes, but some grew in desert. Main center of
diversification for Stellaria is Eurasia, with a center of distribution in
the mountains of E. central Asia. Therefore, due to the importance of these
plant species, we performed a molecular data analysis for this genus. For
this study, we used 65 randomly collected plants from six species in six
provinces. Amplification of genomic DNA using 5 primers produced 70 bands,
of which 62 were polymorphic (84.00%). The obtained high average PIC and MI
values revealed high capacity of ISSR primers to detect polymorphic loci
among Stellaria species. The genetic similarities of six collections were
estimated from 0.69 to 0.85. According to Inter-Simple sequence repeats
(ISSR) markers analysis, S. pallida and S. graminea had the lowest
similarity and the species of S. media and S. pallida had the highest
similarity. The aims of present study are: 1) can ISSR markers identify
Stellaria species, 2) what is the genetic structure of these taxa in Iran,
and 3) to investigate the species inter-relationship? The present study
revealed that ISSR markers can identify the species.
Publisher
National Library of Serbia