Genetic diversity study of Chrysoperla carnea (Neuroptera: Chrysopidae) populations via molecular markers
Author:
Abdolahadi Fatemeh1, Mirmoayedi Alinaghi1, Zaraei Lila2, Jamali Samad1
Affiliation:
1. Department of Plant Protection, Faculty of Agriculture, Campus of Agriculture and natural Resources, Razi university, Kermanshah, Iran 2. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Campus of Agriculture and natural Resources, Razi university, Kermanshah, Iran
Abstract
The objective of this study was to determine the genetic diversity among
Chrysoperla carnea samples collected from different locations of Iran
(including, East-Azerbaijan, West-Azerbaijan, Isfahan, Kerman, Kermanshah,
Lorestan, Mazandaran, Gilan, Hormozgan and Hamedan provinces) using the
Inter simple sequence repeat (ISSR) and mitochondrial (Cytochrome Oxidase I
- COI) molecular markers in 2016-2018. The results showed that a total of 64
bands were produced by ten primers of ISSR markers which among them 43 bands
were polymorphic. The highest and lowest polymorphic percentages belonged to
primer UBC-809 (88.88%) and primer UBC-886 (33.33%), respectively. The
results of cluster analysis based on ISSR marker data divided the samples
into three separate clusters. This grouping was also confirmed by analysis
of molecular variance. According to the results of the analysis of molecular
variance diversity within and among groups was about 84% and 16%,
respectively. In the present study five haplotypes were obtained. The first
haplotype (H1) was common in all populations which can be considered as the
ancestral haplotype, the other haplotypes have been evolved from it. The
novelty of this study is that we report the first time genetic diversity
analysis of family Chrysopidae using ISSR and CO1 markers covering more than
ten provinces and thirty cities of Iran with a full picture of its genetic
diversity. Genetic distance matrix based on Jaccard index indicated low
genetic distance of populations. The results showed that ISSR and CO1
markers have high efficiency in study of genetic diversity in the family
Chrysopidae.
Publisher
National Library of Serbia
Subject
Plant Science,Genetics
Reference38 articles.
1. ABBOT, P. (2001): Individual and population variation in invertebrates revealed by Inter-simple Sequence Repeats (ISSRs). Insect Sci., 1(1):8-21. 2. ANDREWS, C. A (2010): Natural Selection, Genetic Drift, and Gene Flow Do Not Act in Isolation in Natural Populations. Nature Education Knowledge 3(10):5, Available at https://www.nature.com/scitable/knowledge/library/natural-selection-genetic-drift-and-gene-flow-15186648/#(visited 15.December2020) 3. ASOKAN, R., NK., KUMAR, V., KUMAR, H., RANGANATH (2007): Molecular differences in the mitochondrial cytochrome oxidase I (mtCOI) gene and development of a species-specific marker for onion thrips, Thrips tabaci Lindeman and melon thrips, T. palmi Karny (Thysanoptera: Thripidae), vectors of tospoviruses (Bunyaviridae). Bull. Entomol. Res., 97(5):461-470. 4. AVISE, J.C.,J.,ARNOLD, R.M.,BALL,E.,BIRMINGHAM, T.,LAMB,J.E.,NEIGEL, C.A., REEB, N.C., SAUNDERS (1987): Intraspecific phylogeography, the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst., 18:489-522. 5. AWASTHI, AK., G., NAGARAJA, G.,NAIK, S.,KANGINAKUDRU, K.,THANGAVELU, J., NAGARAJU (2004): Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC genetics, (1):156-165.
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